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Antony K. Chen
Ph.D., Assistant Professor
Department of Biomedical Engineering
Peking University

  • Office Add: Integrated Science and Research Building, Room 112
  • Office Tel: +86-10-62768343
  • Lab Add: Integrated Science and Research Building, Room 112
  • Email: chenak@pku.edu.cn

Research Interests:

RNA Imaging Probes, Live-cell Imaging, RNA-Protein Interactions, Virus-Host Interactions, Super-resolution Imaging, Molecular and Nanomedicine


Education

2004-2008   Ph.D.;   University of Pennsylvania                                (in Bioengineering)

2000-2002   M.S.;    University of California, San Diego                       (in Bioengineering)     

1996-2000   B.S.;     University of California, San Diego                          (in Bioengineering)             
 


Major Professional Experiences

2013-Present Assistant Professor, Department of Biomedical Engineering, College of Engineering, Peking University

2010-2013     Postdoc; National Institutes of Health;  Jennifer Lippincott-Schwartz’s lab

2010-2012     Postdoc; National Institute of Standards and Technology;  Anne Plant’s lab

2009/2010     Postdoc; University of Pennsylvania;  Andrew Tsourkas’ lab


Selected Honors

·1000 Young Talent Program, China (2012)

·The US. National Research Council (NRC) Research Associate Fellowship (2010-2012)

·Solomon R. Pollack Award for Excellence in Graduate Bioengineering Research (Best Ph.D. Thesis)   

  (2009)


Major Publications

1. Yu, X., Zhang, Y., Li, T., Ma, Z., Jia, H., Chen, Q., ZHao, Y., Zhai, L., Zhong, R., Li, C., Zou, X., Meng, J., Chen, A.K., Puri, P.L., Chen, M., Zhu, D.* Long Non-coding RNA Linc-RAM Enhances Myogenic Differentiation by Interacting with MyoD. Nat. Commun.2017: 8: 14016

2. Zhao, D., Yang, Y., Qu, N., Chen, M., Ma, Z. Krueger, C.J., Behlke, M.A. and Chen A.K.* Single-Molecule Detection and Tracking of RNA Transcripts in Living Cells Using Phosphorothioate-Optimized 2’-O-Methyl RNA Molecular Beacons. *Corresponding Author. Biomaterials. 2016: 100: 172-183. 

3. Chen, A.K., Sengupta, P., Waki, K., Van Engelenburg, S., Ochiya, T., Ablan, S.D., Freed, E.O. and Lippincott-Schwartz, J.* MicroRNA binding to the HIV-1 Gag protein inhibits Gag assembly and virus production. Proc. Natl. Acad. Sci. U S A., 2014: 111(26):E2676-83.

4. Chung, K.H., Chen, A.K., Anderton, C.R., Bhadriraju, K., Plant, A.L., Bush, B.G., Cook, R.F. and Frank F. Frictional properties of native and functionalized type I collagen thin films. Appl. Phys. Lett. 103 (14), 143703-143703-5.

5. Chen, A.K. * Delrio, F., Peterson, A.W., Chung, K.H., Bhadriraju, K, and Plant, A.L. Cell spreading and proliferation in response to the composition and mechanics of engineered fibrillar extracellular matrices. *Corresponding Author. Biotechnol Bioeng. 2013: 110: 2731-2741.

6. Chen, A.K.* and Tsourkas A. Developing Molecular-Beacon Constructs for Improved Live-Cell Analysis and Imaging of RNA. *Corresponding Author. Am. Biotech. Lab. 2010; 28 (7): 10-13. (Review).

7. Chen, A.K., Davydenko, O., Behlke, M., and Tsourkas A.* Ratiometric Bi-Molecular Beacons: Ratiometric Bimolecular Beacons for the Sensitive Detection of RNA in Single Living Cells. Nucleic Acids Res. 2010; 38 (14): e148.

8. Cheng, Z., Chen, A.K., Lee H-Y., and Tsourkas, A.* Examination of Folate-Targeted Liposomes with Encapsulated Poly(2-Propylacrylic acid) as a pH-responsive Nanoplatform for Cytosolic Drug Delivery. Small. 2010; 6 (13): 1398-401.

9. Chen, A.K., Behlke, M., and Tsourkas, A.* Subcellular Trafficking and Functionality of 2'-O-methyl and 2'-O-methy-phosphorothioate Molecular Beacons. Nucleic Acids Res. 2009; 37 (22): e149.

10. Chen, A.K. and Tsourkas, A.* Imaging RNA in Living Cells with Molecular Beacons: Current Perspectives and Challenges. J. Innov. Opt. Health Sc. 2009; 2 (4): 315-324. (Review) 

11. Chen, A.K., Cheng, Z., Behlke, M., and Tsourkas, A.* “Assessing the Sensitivity of Commercially Available Fluorophores to the Intracellular Environment”. Anal Chem. 2008; 80 (19): 7437-44.

12. Chen, A.K., Behlke, M., and Tsourkas, A.* “Efficient Cytosolic Delivery of Molecular Beacon Conjugates and Flow Cytometric Analysis of Target RNA”. Nucleic Acids Res. 2008; 36 (12): e69.  

13. Elias, D.R., Thorek, D.L., Chen, A.K., Czupryna, J., and Tsourkas, A.* “In vivo Imaging of Cancer Biomarkers Using Activatable Molecular Probes”. Cancer Biomarkers. 2008; 4 (6): 287-305. (Review).

14. Chen, A.K., Behlke, M., and Tsourkas, A.* “Avoiding False-Positive Signals with Nuclease-Vulnerable Molecular Beacons in Single Living Cells”. Nucleic Acids Res. 2007; 35 (16): e105.

15. Chen, A.K., Latz, M.I., Sobolewski, P., and Frangos, J.A.* “Evidence for the Role of G-Proteins in Flow Stimulation of Dinoflagellate Bioluminescence.” Am J Physiol Regul Integr Comp Physiol. 2007; 292(5): R2020-7.

16. Thorek, D.L.J., Chen, A.K., Czupryna, J., and Tsourkas, A.* “Superparamagnetic Iron Oxide Nanoparticle Probes for Molecular Imaging.” Ann Biomed Eng. 2006; Jan;34(1):23-38 (Review).

17. Chen, A.K., Latz, M.I., and Frangos, J.A.* The Use of Dinoflagellate Bioluminescence to Characterize Cell Stimulation in Bioreactors.” Biotechnol Bioeng. 2003; 83(1): 93-103.


Book Chapters

1. Chen, A.K., Rhee, W.J., Bao G. and Tsourkas A.* 2011 Delivery of Molecular Beacons for Live-cell Imaging and Analysis of RNA  RNA Detection and Visualization, Jeffrey E. Gerst, Humana Press, pp 159-174.

2. Crayton, S.H., Chen, A.K., Cheng, Z., Tsourkas, A.* (2010) Molecular Imaging In Comprehensive Biomaterials, edited by Ducheyne, P., Healy, K., Hutmacher, D., Kirkpatrick, J., Elsevier Inc., pp 305-328. 

3. Thorek, D.L.J., Czupryna, J., Chen, A.K., Tsourkas, A.* (2006) Molecular Imaging of Cancer with Superparamagnetic Iron Oxide Nanoparticles. In Cancer Imaging, edited by M.A. Hayat. Elsevier Science/Academic Press, pp 85-95.


Patent Applications

1. Andrew Tsourkas and Antony Chen. Quantitative Molecular Probes, 2007. US Application Number: 12/282.612; International Application Number: PCT/US07/064099.

2. Andrew Tsourkas and Antony Chen. Novel Molecular Beacons. 2009. US Application Number: 61/244,378; International Application Number: PCT/US10/49301.